It comes as Israel presses ahead with its offensive in the famine-stricken city and several countries prepare to recognise a Palestinian state, including the UK.
Health officials at Shifa Hospital said the dead included 14 people killed in a late-night strike on Saturday, which hit a residential block in the southern side of the city.
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Health staff said a nurse who worked at the hospital was among the dead, along with his wife and three children.
The Israeli military, which says it wants to “destroy Hamas’ military infrastructure” and has urged Palestinians to leave, has not given a timeline for its renewed offensive, but there were indications it could take months.
Saturday evening’s strikes come as some prominent Western countries prepare to recognise Palestinian statehood at the gathering of world leaders at the United Nations General Assembly on Monday.
They include the UK, with Prime Minister Keir Starmer set to officially recognise the state of Palestine at an event on Sunday afternoon.
Other countries include France, Canada, Australia, Malta, Belgium and Luxembourg and Portugal.
Ahead of the UN assembly, peace activists in Israel have hailed the planned recognition of a Palestinian state.
On Sunday, a group of more than 60 Jewish and Arab peace and reconciliation organisations, known as It’s Time Coalition, called for an end to the war, the release of the hostages and the recognition of a Palestinian state.
In a video statement, the coalition said: “We refuse to live forever by the sword. The UN decision offers a historic opportunity to move from a death trap to life, from an endless messianic war to a future of security and freedom for both peoples.”
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Yet a ceasefire remains elusive. Israel’s brutal bombardment over the past 23 months has killed more than 65,000 people in Gaza, destroyed vast areas of the strip, displaced around 90% of the population and caused a catastrophic humanitarian crisis, with experts saying Gaza City is experiencing famine.
Israel did not respond to the strikes overnight on Saturday.
In a statement on Sunday, the military said it killed Majed Abu Selmiya, who it said was a sniper for Hamas’s military wing and was preparing to carry out more attacks in the Gaza City area, without providing evidence.
The alleged militant is the brother of the director of Shifa hospital, Dr Mohamed Abu Selmiya, who called the allegations a lie and said Israel was trying to justify the killing of civilians.
Dr Selmiya told The Associated Press that his brother, 57, suffered from hypertension, diabetes, and had vision problems.
As the attacks continue, Israel has ordered hundreds of thousands of Palestinians sheltering in Gaza City to move south to what it calls a humanitarian zone and opened another corridor south of the city for two days this week to allow more people to evacuate.
Palestinians were streaming out of Gaza City by car and on foot, though many are unwilling to be uprooted again, too weak to leave, or unable to afford the cost of moving.
Along the coastal Wadi Gaza route, those too exhausted to continue stopped to catch their breath and give their children a much-needed break from the difficult journey.
Aid groups have warned that forcing thousands of people to evacuate will exacerbate the dire humanitarian crisis. They are appealing for a ceasefire so aid can reach those who need it.
Families of hostages still held by Hamas are also calling for a ceasefire, accusing Israeli Prime Minister Benjamin Netanyahu of condemning their loved ones to death by continuing to fight rather than negotiating an end to the war.
